SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end: Please add a restriction enzyme cut site to each of the original primers to that protein
File:His-tag-primers.png - Wikimedia Commons
Polyhistidine-tag - Wikipedia
pET-28a - $162.00
Primer
SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a
Structural Biochemistry/Proteins/Purification/Affinity chromatography - Wikibooks, open books for an open world
pCMV / hygro-Positive Control Vector (C-terminal Fc-His tag) | SinoBiological
Harms Lab | Cloning of S100 with N-terminal TEV cleavage 6xHis-Tag
biotechrabbit | RTS Wheat Germ LinTempGen Set, His6-tag - Vectors and Linear Template Kits - Rapid Translation Systems (RTS) - Cell-free Protein Synthesis - Products | leap and lead
Polyhistidine-Tag - an overview | ScienceDirect Topics
Addgene: Sfp pet29b C-terminal His Tag
His-Tag | Definition & Data
NHT-H2IN amplification primers. PCR primers used to amplify the HTLV-2... | Download Scientific Diagram
Typical design of N- or C-terminal His-tagged protein expression... | Download Scientific Diagram
Design Primers with a His-tag for YGOI and Insert to Plasmid - YouTube
First primer F/R sets (N terminus 6x His tag) (each 5 nmole)
His-Tag | Definition & Data
SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein
Enzyme Purification by Affinity Column for Tagged Enzymes
Harms Lab | Cloning of S100 with N-terminal TEV cleavage 6xHis-Tag
His Tagged Protein Purification | Sino Biological
Addgene: pET28a His tag-FRB-Venus C
pET-52 3C/LIC Vector Kit - Novagen | 71571
RTS™ 100 E. coli LinTempGenSet, His-tag Manual
Cloning of scFv Fragments | Antibody Design Labs
pCMV3-C-His Negative Control Vector (C-terminal His-tagged) | SinoBiological
