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![PDF] Optimization of the annealing temperature for DNA amplification in vitro. | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/3c10ed8f9f0e62facd310d28555ed60e3982d6c4/2-TableI-1.png "PDF] Optimization of the annealing temperature for DNA amplification in vitro. | Semantic Scholar")
PDF] Optimization of the annealing temperature for DNA amplification in vitro. | Semantic Scholar
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€
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